胃粘膜瓣成型预防食管胃吻合术后反流的观察

目的:介绍胃粘膜瓣成型预防食管胃吻合术后反流的临床应用.方法:食管、贲门癌50例,行部分食管部分胃切除,用吻合器作食管胃吻合后行胃粘膜瓣成型.结果:成型组术后无反流症状,上消化道造影及放射性核素显像表明该手术方法抗反流作用明显.食管镜检查示胃粘膜瓣成型组吻合口粘膜充血、水肿、糜烂、溃疡较常规手术组明显减轻.结论:胃粘膜瓣成型术可有效预防食管胃吻合术后胃食管反流.     

以BAFF/APRIL为靶点药物治疗系统性红斑狼疮临床研究进展

系统性红斑狼疮(SLE)是一种以致病性自身抗体和免疫复合物形成并介导器官、组织损伤的自身免疫性疾病,近年来不少国内外学者发现B淋巴细胞在SLE的发病中扮演了重要的角色,本文主要对以BAFF/APRIL为靶点的药物治疗SLE的临床试验进展进行综述。     

叶酸对DNMTl和MeCP2在宫颈癌细胞中表达调节的实验研究

目的探讨宫颈癌细胞中叶酸对DNA甲基转移酶1(DNMTl)和甲基化CpG岛结合蛋白2(MeCP2)表达的调节作用。方法采用体外实验研究方法,对宫颈癌细胞casl(i(HPVl6阳性)和C33A(HPV阴性)进行不同浓度(10、50、100、500、750、1000 I．tg/m1)叶酸干预,分别采用Western blot和real-time PCR方法检测两种细胞中DNMTl和MeCP2蛋白的表达量和mRNA水平。结果随着叶酸水平的升高,两种细胞的生长抑制率(C33A:r=0．984,Pr=0．978,P=0．002)和凋亡率(C33A：r=0．989,P<0．001;Caski：r=0．994,Pr=0．914,Pr=0．859,P=0．003)及MeCP2蛋白表达(C33A：r=0．830,P=0．005;Caski：r=0．981,P<0．001)均呈逐渐降低趋势,而DNMTl和MeCP2 mRNA的Ct比值在两种细胞中的变化均无统计学意义(P>O．05)。相同叶酸浓度下,DNMTl蛋白和mRNA表达水平在Caski细胞均较C33A细胞为高,而MeCP2蛋白和mRNA表达水平则在C33A细胞较Caski细胞普遍为高。结论补充叶酸可有效抑制宫颈癌细胞的增殖,促进凋亡,逆转DNMTl和MeCP2蛋白的异常表达;HPVl6感染与DNMTl功能异常对宫颈癌的发生可能有协同效应。     

The Case-Control Study Between DDAH2 Polymorphism and Coronary Heart Disease

Background and Aim Asymmetric dimethylarginine(ADMA),an endogenous inhibitor of nitric oxide synthase(NOS),has been shown to be an independent predictor of coronary heart disease(CHD).Dimethylarginine dimethylaminohydrolase-2(DDAH2) promotes the metabolism of ADMA and plays a key role in formation of the atherosclerosis.We hypothesized that genetic variation inDDAH2 gene might alter the susceptibility to CHD.Methods We tested our hypothesis in a case-control studies.We used ahaplotype-tagging single nucleotide polymorphisms(SNP) approach to identify tag SNP in DDAH2.The SNP were genotyped by poly-merase chain reaction-restriction fragment length polymorphism(PCR-RFLP) and ligase detection reaction(LDR)-sequencing in 1650patients with CHD and 1920 control subjects.Results Apromoter variant-449C/G(rs805305) and -1415G/A(rs2272592)in DDAH2 was identified in the region containing DDAH2.The frequency of those polymorphism were consistent with the lawof Har-dy-Weinberg.The frequency of rs805305 CG +GG or G allele was not significantly different between CHD and wild-type genotype(OR: 0.667,95%CI: 0.374 to 1.187,p>0.05).The frequency of rs2272592 GA +AA or A allele also showed no significant difference between CHD and wild-type genotype(OR: 1.420,95%CI: 0.899 to 2.242,p>0.05).No association was observed be-tween the DDAH2 variant and CHD.These results was independent of age,gender,hypertension,diabetes and hyperlipidemia.Conclusions Our results suggest that although DDAH2/ADMA pathway acts as a critical regulator of coronary atherosclerotic heartdisease,the DDAH2 common variant may not predict the susceptibility to CHD in Chinese population.